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microbiospin p 30 chromatography columns  (Bio-Rad)


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    Structured Review

    Bio-Rad microbiospin p 30 chromatography columns
    Microbiospin P 30 Chromatography Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1030 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microbiospin+p+30+chromatography+columns/Bio-Spin+Chromatography+Columns/pmc08973846__supp_gad__349172__121_Supplemental_Figures_-69-16-20
    Average 96 stars, based on 1030 article reviews
    microbiospin p 30 chromatography columns - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Nick Translation:

    Article Title: Nucleolar-based Dux repression is essential for embryonic two-cell stage exit
    Article Snippet: The genomic region of Dux locus was detected using fosmid probes (WI1-1484C16; Genomic Coordinates: chr10:57682337-57718286) from BACPAC Resources Center (https://bacpacresources.org/; California, USA). .. Probes were labelled with tetramethyl-rhodamine-5-dUTP (Roche) by nick translation (Roche), and purified from unincorporated nucleotides using MicroBioSpin P-30 chromatography columns (Bio-Rad). ..

    Article Title: Genome architecture mapping
    Article Snippet: .. Probes were labelled with digoxigenin-11-dUTP, fluorescein-12-dUTP or tetramethyl-rhodamine-5-dUTP by nick translation (Roche), and separated from unincorporated nucleotides using MicroBioSpin P-30 chromatography columns (BioRad, Hertfordshire, UK). .. The signal of rhodamine-labelled probes was amplified with rabbit anti-rhodamine antibodies (2 h; 1:500; Invitrogen) and Cyanine3-conjugated donkey antibodies against rabbit IgG (1 h; 1:1000; Jackson ImmunoResearch Laboratories).

    Article Title: Polycomb Associates Genome-wide with a Specific RNA Polymerase II Variant, and Regulates Metabolic Genes in ESCs
    Article Snippet: BAC and fosmid probes were obtained from BACPAC Resources (California, USA). .. Probes were labelled with tetramethyl- rhodamine-5-dUTP or digoxigenin-11-dUTP by nick translation (Roche), and purified using MicroBioSpin P-30 chromatography columns (BioRad). .. The signal of rhodamine-labelled probes was amplified with rabbit anti-rhodamine antibodies (2h; 1:500; Invitrogen) and Cyanine3-conjugated donkey antibodies against rabbit IgG (1h; 1:1000; Jackson ImmunoResearch Laboratories).

    Article Title: Complex multi-enhancer contacts captured by Genome Architecture Mapping (GAM)
    Article Snippet: .. Probes were labelled with tetramethyl-rhodamine-5-dUTP by nick translation (Roche), and separated from unincorporated nucleotides using MicroBioSpin P-30 chromatography columns (BioRad, Hertfordshire, UK). .. Hybridization mixtures contained 50% deionised formamide (Sigma), 2xSSC, 10% dextran sulphate, 50 mM phosphate buffer (pH 7.0), 1 μg/μl Cot1 DNA, 2 μg/μl salmon sperm DNA and 2-4 μl nick-translated probe.

    Article Title: Active and poised promoter states drive folding of the extended HoxB locus in mouse embryonic stem cells.
    Article Snippet: .. Probes were labeled with digoxigenin-11-dUTP, fluorescein-12-dUTP or tetramethyl-rhodamine-5-dUTP by nick translation (Roche), and purified from unincorporated nucleotides using MicroBioSpin P-30 chromatography columns (Bio–Rad). ..

    Purification:

    Article Title: Nucleolar-based Dux repression is essential for embryonic two-cell stage exit
    Article Snippet: The genomic region of Dux locus was detected using fosmid probes (WI1-1484C16; Genomic Coordinates: chr10:57682337-57718286) from BACPAC Resources Center (https://bacpacresources.org/; California, USA). .. Probes were labelled with tetramethyl-rhodamine-5-dUTP (Roche) by nick translation (Roche), and purified from unincorporated nucleotides using MicroBioSpin P-30 chromatography columns (Bio-Rad). ..

    Article Title: Polycomb Associates Genome-wide with a Specific RNA Polymerase II Variant, and Regulates Metabolic Genes in ESCs
    Article Snippet: BAC and fosmid probes were obtained from BACPAC Resources (California, USA). .. Probes were labelled with tetramethyl- rhodamine-5-dUTP or digoxigenin-11-dUTP by nick translation (Roche), and purified using MicroBioSpin P-30 chromatography columns (BioRad). .. The signal of rhodamine-labelled probes was amplified with rabbit anti-rhodamine antibodies (2h; 1:500; Invitrogen) and Cyanine3-conjugated donkey antibodies against rabbit IgG (1h; 1:1000; Jackson ImmunoResearch Laboratories).

    Article Title: Active and poised promoter states drive folding of the extended HoxB locus in mouse embryonic stem cells.
    Article Snippet: .. Probes were labeled with digoxigenin-11-dUTP, fluorescein-12-dUTP or tetramethyl-rhodamine-5-dUTP by nick translation (Roche), and purified from unincorporated nucleotides using MicroBioSpin P-30 chromatography columns (Bio–Rad). ..

    Chromatography:

    Article Title: Nucleolar-based Dux repression is essential for embryonic two-cell stage exit
    Article Snippet: The genomic region of Dux locus was detected using fosmid probes (WI1-1484C16; Genomic Coordinates: chr10:57682337-57718286) from BACPAC Resources Center (https://bacpacresources.org/; California, USA). .. Probes were labelled with tetramethyl-rhodamine-5-dUTP (Roche) by nick translation (Roche), and purified from unincorporated nucleotides using MicroBioSpin P-30 chromatography columns (Bio-Rad). ..

    Article Title: Genome architecture mapping
    Article Snippet: .. Probes were labelled with digoxigenin-11-dUTP, fluorescein-12-dUTP or tetramethyl-rhodamine-5-dUTP by nick translation (Roche), and separated from unincorporated nucleotides using MicroBioSpin P-30 chromatography columns (BioRad, Hertfordshire, UK). .. The signal of rhodamine-labelled probes was amplified with rabbit anti-rhodamine antibodies (2 h; 1:500; Invitrogen) and Cyanine3-conjugated donkey antibodies against rabbit IgG (1 h; 1:1000; Jackson ImmunoResearch Laboratories).

    Article Title: Polycomb Associates Genome-wide with a Specific RNA Polymerase II Variant, and Regulates Metabolic Genes in ESCs
    Article Snippet: BAC and fosmid probes were obtained from BACPAC Resources (California, USA). .. Probes were labelled with tetramethyl- rhodamine-5-dUTP or digoxigenin-11-dUTP by nick translation (Roche), and purified using MicroBioSpin P-30 chromatography columns (BioRad). .. The signal of rhodamine-labelled probes was amplified with rabbit anti-rhodamine antibodies (2h; 1:500; Invitrogen) and Cyanine3-conjugated donkey antibodies against rabbit IgG (1h; 1:1000; Jackson ImmunoResearch Laboratories).

    Article Title: Complex multi-enhancer contacts captured by Genome Architecture Mapping (GAM)
    Article Snippet: .. Probes were labelled with tetramethyl-rhodamine-5-dUTP by nick translation (Roche), and separated from unincorporated nucleotides using MicroBioSpin P-30 chromatography columns (BioRad, Hertfordshire, UK). .. Hybridization mixtures contained 50% deionised formamide (Sigma), 2xSSC, 10% dextran sulphate, 50 mM phosphate buffer (pH 7.0), 1 μg/μl Cot1 DNA, 2 μg/μl salmon sperm DNA and 2-4 μl nick-translated probe.

    Article Title: Active and poised promoter states drive folding of the extended HoxB locus in mouse embryonic stem cells.
    Article Snippet: .. Probes were labeled with digoxigenin-11-dUTP, fluorescein-12-dUTP or tetramethyl-rhodamine-5-dUTP by nick translation (Roche), and purified from unincorporated nucleotides using MicroBioSpin P-30 chromatography columns (Bio–Rad). ..

    Labeling:

    Article Title: Active and poised promoter states drive folding of the extended HoxB locus in mouse embryonic stem cells.
    Article Snippet: .. Probes were labeled with digoxigenin-11-dUTP, fluorescein-12-dUTP or tetramethyl-rhodamine-5-dUTP by nick translation (Roche), and purified from unincorporated nucleotides using MicroBioSpin P-30 chromatography columns (Bio–Rad). ..



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